Modifying Enzymes
Apexbio Technology LLC DNase I (RNase-free),1000U
DNase I (RNase-free) is an endonuclease that digests single- or double- stranded DNA, generating dinucleotide, trinucleotide, and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. The activity of DNase I (RNase-free) dependent on Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, DNase I (RNase-free) can randomly cleaves arbitrary sites of double-stranded DNA; in the presence of Mn2+, DNase I (RNase-free) can simultaneously recognize both strands of DNA and cleaves at nearly same sites. DNase I (RNase-free) can act on single stranded DNA, double stranded DNA, chromatin, and RNA: DNA hybrid strands. DNase I (RNase-free) is suitable for RNA extraction, in vitro transcription, and removal of DNA for RT-PCR experiments.Other sizes are also available. Please inqury us for quote.
Discovery Life Sciences HUMAN CYP1A2+REDUCTASE 0.5NMOL
Human CYP1A2 + P450 Reductase SUPERSOMES 0.5 nmole cytochrome P450 in 0.5mL This activity is catalyzed by CYP1A2 which is expressed from human CYP1A2 cDNA using a baculovirus expression system
Discovery Life Sciences HUM CYP2C19+REDUCTASE+B5 0.5NM
Human CYP2C19 + P450 Reductase + Cytochrome b5 SUPERSOMES 0.5 nmole cytochrome P450 in 0.5mL This activity is catalyzed by CYP2C19 expressed from human CYP2C19 cDNA using a baculovirus expression system
Supply Solutions Sigma Aldrich - Liberase Th Research Grade 100 Mg * Third Party Item
Sigma Aldrich - Liberase Th Research Grade 100 Mg * Third Party Item
INTACT GENOMICS INC Endonuclease IV (Nfo) 2000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
INTACT GENOMICS INC Exonuclease III 10000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios (3-5).Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Medchemexpress LLC Fibrinogen (bovine) | 9001-32-5 | 92.0% | ≈340 kDa | 100MG
Bovine fibrinogen is a plasma glycoprotein supplied as a white to off-white powder for research applications in coagulation, thrombosis, and vascular biology. It is converted to fibrin by thrombin and is used in in vitro clotting assays and studies of inflammatory signaling in endothelial cells.
- White to off-white powder; soluble plasma glycoprotein.
- Used to study blood clotting, fibrin formation, thrombosis, and vascular inflammation.
- Activated by thrombin to assemble fibrin clots.
- Suitable for in vitro clotting assays and endothelial cell signaling studies.
- Stored per certificate of analysis; typically kept frozen for long-term storage (e.g., -20°C).
- Documentation available: datasheet, certificate of analysis, safety data sheet, and handling instructions.
- CAS number: 9001-32-5.
Sigma Aldrich Fine Chemicals Biosciences Thrombin from human plasma lyophilized powder, Suitable for routine use in the thrombin time test | 9002-04-4 | MFCD00082072 | 10VL
Thrombin from human plasma lyophilized powder, Suitable for routine use in the thrombin time test | 9002-04-4 | MFCD00082072 | 10VL
Cayman Chemical Acetyl-Coenzyme A-d3sodIum 5mg
An internal standard for the quantification of acetyl-coenzyme A by GC- or LC-MS
Cytoskeleton, Inc. CELL PERMEABLE C3 TRANS FERASE
Cell Permeable Rho Inhibitor (C3 Trans based)) 1 x 20 ug
Cayman Chemical ANTIBODY KU-0058948 25mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
![Revvity Health Sciences Inc Acetyl Coenzyme A, [acetyl-1-<sup>14</sup>C]-, 10 µCi (370 kBq)](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502098430.jpg-250.jpg)
Revvity Health Sciences Inc Acetyl Coenzyme A, [acetyl-1-14C]-, 10 µCi (370 kBq)
Acetyl Coenzyme A, [acetyl-1-14C]-, 10 µCi (370 kBq)
Crescent Chemical Co Inc RIBONUCLEASE A FRM BOVINE PANC
Ribonuclease A from bovine pancreas min. 80 Kunitz units/mg lyophil. An endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. This preparation is salt-free, protease-free and chromatographically homogeneous.RNase A content: min. 90 % by ion exchange chromatography. DNa